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Title: Transient expression in Physarum of a chloramphenicol acetyltransferase gene under the control of actin gene promoters
Authors: Burland, T.
Bailey, Juliet
Adam, L.
Mukhopadhyay, M.
Dove, W.
Pallotta, D.
First Published: Apr-1992
Publisher: Springer Verlag
Citation: Current Genetics, 1992, 21 (4-5), pp. 393-398
Abstract: We cloned and sequenced two actin promoters from Physarum, and constructed plasmids carrying these promoters upstream of a bacterial chloramphenicol acetyltransferase (cat) gene. We then tested the plasmids for their ability to express cat in Physarum amoebae. We present reliable methods for introducing plasmid DNA into Physarum amoebae by electroporation, and show that expression of the cat gene in amoebae occurs in the presence, but not the absence, of one or the other Physarum actin promoter.
DOI Link: 10.1007/BF00351700
ISSN: 0172-8083
eISSN: 1432-0983
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Biology

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