Please use this identifier to cite or link to this item:
|Title:||Stable, selectable, integrative DNA transformation in Physarum|
|Authors:||Burland, T. G.|
Dove, W. F.
|Citation:||Gene, 1993, 132 (2), pp. 207-212|
|Abstract:||The Physarum polycephalum actin promoter, PardC, can drive transient expression of heterologous genes in Physarum amoebae. The hph gene, encoding hygromycin (Hy) phosphotransferase, can confer resistance to Hy on a broad spectrum of organisms. When PardC is translationally fused to hph and transformed into yeasts on high-copy-number vectors, the yeasts become Hy resistant (Hy[superscript: R]), showing that PardC-hph is a functional, selectable genetic element. To establish a stable transformation system for Physarum, we electroporated plasmids bearing PardC-hph into Physarum amoebae and then selected for Hy[superscript: R] transformants. We show that Hy[superscript: R] amoebae arise upon the stable integration of PardC-hph into the nuclear genome in single copy. These results establish a transformation system that can be used to add plasmidborne genetic information to Physarum.|
|Appears in Collections:||Published Articles, Dept. of Genetics|
Files in This Item:
There are no files associated with this item.
Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.