Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/11741
Title: Targeted enrichment of genomic DNA regions for next-generation sequencing
Authors: Mertes, Florian
ElSharawy, Abdou
Sauer, Sascha
van Helvoort, Joop M. L. M.
Nilsson, Mats
Lehrach, Hans
Brookes, Anthony J.
First Published: 25-Nov-2011
Publisher: Oxford University Press (OUP)
Citation: Briefings in Functional Genomics, 2011, 10 (6), pp. 374-386
Abstract: In this review, we discuss the latest targeted enrichment methods and aspects of their utilization along with second-generation sequencing for complex genome analysis. In doing so, we provide an overview of issues involved in detecting genetic variation, for which targeted enrichment has become a powerful tool. We explain how targeted enrichment for next-generation sequencing has made great progress in terms of methodology, ease of use and applicability, but emphasize the remaining challenges such as the lack of even coverage across targeted regions. Costs are also considered versus the alternative of whole-genome sequencing which is becoming ever more affordable. We conclude that targeted enrichment is likely to be the most economical option for many years to come in a range of settings.
DOI Link: 10.1093/bfgp/elr033
ISSN: 2041-2649
eISSN: 2041-2657
Links: http://hdl.handle.net/2381/11741
https://academic.oup.com/bfg/article-lookup/doi/10.1093/bfgp/elr033
Version: Publisher Version
Type: Journal Article
Rights: Copyright © the authors, 2011. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-commercial License (https://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted use, distribution, and reproduction in any medium non-commercially, provided the original author and source are credited.
Appears in Collections:Published Articles, Dept. of Genetics

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