Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/12698
Title: Stochastic noise and synchronisation during dictyostelium aggregation make cAMP oscillations robust.
Authors: Kim, Jongrae
Heslop-Harrison, Pat
Postlethwaite, Ian
Bates, Declan G.
First Published: 9-Nov-2007
Publisher: Public Library of Science for International Society for Computational Biology (ISCB)
Citation: PLoS Computational Biology, 2007, 3 (11), pp. e218-e218
Abstract: Stable and robust oscillations in the concentration of adenosine 3', 5'-cyclic monophosphate (cAMP) are observed during the aggregation phase of starvation-induced development in Dictyostelium discoideum. In this paper we use mathematical modelling together with ideas from robust control theory to identify two factors which appear to make crucial contributions to ensuring the robustness of these oscillations. Firstly, we show that stochastic fluctuations in the molecular interactions play an important role in preserving stable oscillations in the face of variations in the kinetics of the intracellular network. Secondly, we show that synchronisation of the aggregating cells through the diffusion of extracellular cAMP is a key factor in ensuring robustness of the oscillatory waves of cAMP observed in Dictyostelium cell cultures to cell-to-cell variations. A striking and quite general implication of the results is that the robustness analysis of models of oscillating biomolecular networks (circadian clocks, Ca(2+) oscillations, etc.) can only be done reliably by using stochastic simulations, even in the case where molecular concentrations are very high.
Stable and robust oscillations in the concentration of adenosine 3′, 5′-cyclic monophosphate (cAMP) are observed during the aggregation phase of starvation-induced development in Dictyostelium discoideum. In this paper we use mathematical modelling together with ideas from robust control theory to identify two factors which appear to make crucial contributions to ensuring the robustness of these oscillations. Firstly, we show that stochastic fluctuations in the molecular interactions play an important role in preserving stable oscillations in the face of variations in the kinetics of the intracellular network. Secondly, we show that synchronisation of the aggregating cells through the diffusion of extracellular cAMP is a key factor in ensuring robustness of the oscillatory waves of cAMP observed in Dictyostelium cell cultures to cell-to-cell variations. A striking and quite general implication of the results is that the robustness analysis of models of oscillating biomolecular networks (circadian clocks, Ca2+ oscillations, etc.) can only be done reliably by using stochastic simulations, even in the case where molecular concentrations are very high.
DOI Link: 10.1371/journal.pcbi.0030218
ISSN: 1553-734X
eISSN: 1553-7358
Links: http://hdl.handle.net/2381/12698
http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.0030218
Type: Journal Article
Rights: Copyright 2007 Kim et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY.
Appears in Collections:Published Articles, Dept. of Engineering

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