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dc.contributor.authorHughes, Reanneen_GB
dc.contributor.authorAndrew, Peter W.en_GB
dc.contributor.authorKilvington, Simonen_GB
dc.identifier.citationApplied and Environmental Microbiology, 2003, 69 (5), pp.2563-2567en_GB
dc.description.abstractThe activity of H[subscript 2]O[subscript 2] against the resistant cyst stage of the pathogenic free-living amoeba Acanthamoeba was enhanced by the addition of KI and either horseradish peroxidase or soybean peroxidase or, to a lesser degree, lactoperoxidase. This resulted in an increase in the cysticidal activity of 3% (wt/vol) H[subscript 2]O[subscript 2], and there was >3-log killing in 2 h, compared with the 6 h required for comparable results with the peroxide solution alone (P < 0.05). With 2% H[subscript 2]O[subscript 2], enhancement was observed at all time points (P < 0.05), and total killing of the cyst inoculum occurred at 4 h, compared with 6 h for the peroxide alone. The activity of sublethal 1% H[subscript 2]O[subscript 2] was enhanced to give 3-log killing after 8 h of exposure (P < 0.05). No enhancement was obtained when KCl or catalase was used as a substitute in the reaction mixtures. The H[subscript 2]O[subscript 2] was not neutralized in the enhanced system during the experiments. However, in the presence of a platinum disk used to neutralize H[subscript 2]O[subscript 2] in contact lens care systems, the enhanced 2% H[subscript 2]O[subscript 2] system gave 2.8-log killing after 6 h or total cyst killing by 8 h, and total neutralization of the H[subscript 2]O[subscript 2] occurred by 4 h. In contrast, 2% H[subscript 2]O[subscript 2] alone resulted in <0.8-log killing of cysts in the presence of the platinum disk due to rapid (<1 h) neutralization of the peroxide. Our observations could result in significant improvement in the efficacy of H[subscript 2]O[subscript 2] contact lens disinfection systems against Acanthamoeba cysts and prevention of acanthamoeba keratitis.-
dc.publisherAmerican Society for Microbiology-
dc.rightsCopyright © 2013 by the American Society for Microbiology. Deposited with reference to the publisher’s archiving policy available on the SHERPA/RoMEO website.-
dc.titleEnhanced killing of Acanthamoeba cysts with a plant peroxidase-hydrogen peroxide-halide antimicrobial systemen_GB
dc.description.versionPublisher Version-
dc.relation.raeRAE 2007-
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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