Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/13860
Title: Immuno-Slot Blot Assay for Detection of UVR-Mediated DNA Damage.
Authors: Karbaschi, M
Brady, NJ
Evans, MD
Cooke, MS
First Published: 2012
Citation: METHODS MOL BIOL, 2012, 920, pp. 163-175
Abstract: Solar ultraviolet radiation (UVR), through the formation of DNA photolesions, is the primary cause of most skin cancers. A better understanding of the mechanisms of UVR-induced DNA damage may help prevent skin cancer and this may be achieved using methods to quantify DNA damage. The immuno-slot blot (ISB) method is routinely used for detection and quantification of any heat- and alkali-stable DNA adducts for which a sufficiently specific monoclonal antibody is available. The main steps in ISB are fragmentation and denaturation of the DNA, immobilization of DNA to a nitrocellulose filter, incubation with primary antibody against a specific DNA adduct, incubation with an enzyme-linked secondary antibody and finally chemiluminescence detection and quantification of the DNA adducts.
DOI Link: 10.1007/978-1-61779-998-3_12
eISSN: 1940-6029
Links: http://hdl.handle.net/2381/13860
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Cancer Studies and Molecular Medicine

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