Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/13908
Title: Primary human airway epithelial cell-dependent inhibition of human lung mast cell degranulation.
Authors: Martin, N.
Ruddick, A.
Arthur, G. K.
Wan, H.
Woodman, L.
Brightling, C. E.
Jones, D. J.
Pavord, I. D.
Bradding, P.
First Published: 27-Aug-2012
Citation: PLoS ONE, 2012, 7 (8), pp. e43545-e43545
Abstract: Chronic mast cell activation is a characteristic feature of asthma. BEAS-2B human airway epithelial cells (AEC) profoundly inhibit both constitutive and IgE-dependent human lung mast cell (HLMC) histamine release. The aim of this study was to examine the regulation of HLMC degranulation by primary AEC from healthy and asthmatic subjects, and investigate further the inhibitory mechanism. Methods: HLMC were co-cultured with both BEAS-2B and primary AEC grown as monolayers or air-liquid interface (ALI) cultures. Results: Both constitutive and IgE-dependent HLMC histamine release were attenuated by BEAS-2B, primary AEC monolayers and ALI cultures. This occurred in the absence of HLMC-AEC contact indicating the presence of a soluble factor. Unlike healthy ALI AEC, asthmatic ALI-AEC did not significantly reduce constitutive histamine release. AEC inhibitory activity was transferable in primary AEC monolayer supernatant, but less active than with Transwell co-culture, suggesting that the inhibitory factor was labile. The AEC inhibitory effects were attenuated by both AEC wounding and pertussis toxin, indicating the involvement of a G0/Gi receptor coupled mechanism. Solid phase extraction of lipids (<10 kDa) removed the AEC inhibitory activity. The lipid derivatives resolvin D1 and D2 and lipoxin A4 attenuated HLMC histamine release in a dose-dependent fashion but were not detectable in co-culture supernatants. Conclusions: Primary AEC suppress HLMC constitutive and IgE-dependent histamine secretion through the release of a soluble, labile lipid mediator(s) that signals through the G0/Gi receptor coupled mechanism. Manipulation of this interaction may have a significant therapeutic role in asthma.
DOI Link: 10.1371/journal.pone.0043545
eISSN: 1932-6203
Links: http://hdl.handle.net/2381/13908
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0043545
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © Martin et al, 2012. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Appears in Collections:Published Articles, Dept. of Cancer Studies and Molecular Medicine

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