Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/14140
Title: MicroRNA and tasiRNA diversity in mature pollen of Arabidopsis thaliana.
Authors: Grant-Downton, R.
Le Trionnaire, Gael
Schmid, Ralf
Rodriguez-Enriquez, J.
Hafidh, S.
Mehdi, S.
Twell, D.
Dickinson, H.
First Published: 30-Dec-2009
Publisher: BioMed Central Ltd
Citation: BMC Genomics, 2009, 10:643
Abstract: Background:New generation sequencing technology has allowed investigation of the small RNA populations of flowering plants at great depth. However, little is known about small RNAs in their reproductive cells, especially in post-meiotic cells of the gametophyte generation. Pollen - the male gametophyte - is the specialised haploid structure that generates and delivers the sperm cells to the female gametes at fertilisation. Whether development and differentiation of the male gametophyte depends on the action of microRNAs and trans-acting siRNAs guiding changes in gene expression is largely unknown. Here we have used 454 sequencing to survey the various small RNA populations present in mature pollen of Arabidopsis thaliana. Results:In this study we detected the presence of 33 different microRNA families in mature pollen and validated the expression levels of 17 selected miRNAs by Q-RT-PCR. The majority of the selected miRNAs showed pollen-enriched expression compared with leaves. Furthermore, we report for the first time the presence of trans-acting siRNAs in pollen. In addition to describing new patterns of expression for known small RNAs in each of these classes, we identified 7 putative novel microRNAs. One of these, ath-MIR2939, targets a pollen-specific F-box transcript and we demonstrate cleavage of its target mRNA in mature pollen. Conclusions:Despite the apparent simplicity of the male gametophyte, comprising just two different cell types, pollen not only utilises many miRNAs and trans-acting siRNAs expressed in the somatic tissues but also expresses novel miRNAs.
DOI Link: 10.1186/1471-2164-10-643
eISSN: 1471-2164
Links: http://hdl.handle.net/2381/14140
http://www.biomedcentral.com/1471-2164/10/643
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2009 Grant-Downton et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Appears in Collections:Published Articles, Dept. of Biology

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