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Title: In situ analysis of gene expression in plants.
Authors: Drea, S
Derbyshire, P
Koumproglou, R
Dolan, L
Doonan, JH
Shaw, P
First Published: 2009
Citation: METHODS MOL BIOL, 2009, 513, pp. 229-242
Abstract: In the post-genomic era, it is necessary to adapt methods for gene expression and functional analyses to more high-throughput levels of processing. mRNA in situ hybridization (ISH) remains a powerful tool for obtaining information regarding a gene's temporal and spatial expression pattern and can therefore be used as a starting point to define the function of a gene or a whole set of genes. We have deconstructed 'traditional' ISH techniques described for a range of organisms and developed protocols for ISH that adapt and integrate a degree of automation to standardized and shortened protocols. We have adapted this technique as a high-throughput means of gene expression analysis on wax-embedded plant tissues and also on whole-mount tissues. We have used wax-embedded wheat grains and Arabidopsis floral meristems and whole-mount Arabidopsis roots as test systems and show that it is capable of highly parallel processing.
DOI Link: 10.1007/978-1-59745-427-8_12
ISSN: 1064-3745
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Biology

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