Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/14290
Title: A plant germline-specific integrator of sperm specification and cell cycle progression.
Authors: Brownfield, Lynette
Hafidh, Said
Borg, Michael
Sidorova, Anna
Mori, T.
Twell, David
First Published: 20-Mar-2009
Publisher: Public Library of Science
Citation: PLoS Genetics, 2009, 5 (3), pp. e1000430-e1000430
Abstract: The unique double fertilisation mechanism in flowering plants depends upon a pair of functional sperm cells. During male gametogenesis, each haploid microspore undergoes an asymmetric division to produce a large, non-germline vegetative cell and a single germ cell that divides once to produce the sperm cell pair. Despite the importance of sperm cells in plant reproduction, relatively little is known about the molecular mechanisms controlling germ cell proliferation and specification. Here, we investigate the role of the Arabidopsis male germline-specific Myb protein DUO POLLEN1, DUO1, as a positive regulator of male germline development. We show that DUO1 is required for correct male germ cell differentiation including the expression of key genes required for fertilisation. DUO1 is also necessary for male germ cell division, and we show that DUO1 is required for the germline expression of the G2/M regulator AtCycB1;1 and that AtCycB1:1 can partially rescue defective germ cell division in duo1. We further show that the male germline-restricted expression of DUO1 depends upon positive promoter elements and not upon a proposed repressor binding site. Thus, DUO1 is a key regulator in the production of functional sperm cells in flowering plants that has a novel integrative role linking gametic cell specification and cell cycle progression.
DOI Link: 10.1371/journal.pgen.1000430
ISSN: 1553-7390
eISSN: 1553-7404
Links: http://hdl.handle.net/2381/14290
http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1000430
Type: Journal Article
Rights: Copyright: © 2009 Brownfield et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Appears in Collections:Published Articles, Dept. of Biology

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