Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/14379
Title: Disassembly of the JIP1/JNK molecular scaffold by caspase-3-mediated cleavage of JIP1 during apoptosis.
Authors: Vaishnav, M
MacFarlane, M
Dickens, M
First Published: 15-Apr-2011
Citation: EXP CELL RES, 2011, 317 (7), pp. 1028-1039
Abstract: We report here the cleavage of the c-Jun N-terminal Kinase (JNK) pathway scaffold protein, JNK Interacting Protein-1 (JIP1), by caspases during both Tumour Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL) and staurosporine-induced apoptosis in HeLa cells. During the initiation of apoptosis, maximal JNK activation is observed when JIP1 is intact, whereas cleavage of JIP1 correlates with JNK inactivation and progression of apoptosis. JIP1 is cleaved by caspase-3 at two sites, leading to disassembly of the JIP1/JNK complex. Inhibition of JIP1 cleavage by the caspase-3 inhibitor DEVD.fmk inhibits this disassembly, and is accompanied by sustained JNK activation. These data suggest that TRAIL and staurosporine induce JNK activation in a caspase-3-independent manner and that caspase-3-mediated JIP1 cleavage plays a role in JNK inactivation via scaffold disassembly during the execution phase of apoptosis. Caspase-mediated cleavage of JIP scaffold proteins may therefore represent an important mechanism for modulation of JNK signalling during apoptotic cell death.
DOI Link: 10.1016/j.yexcr.2011.01.011
eISSN: 1090-2422
Links: http://hdl.handle.net/2381/14379
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Biochemistry

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