Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/14402
Title: Synemin may function to directly link muscle cell intermediate filaments to both myofibrillar Z-lines and costameres.
Authors: Bellin, RM
Huiatt, TW
Critchley, DR
Robson, RM
First Published: 24-Aug-2001
Citation: J BIOL CHEM, 2001, 276 (34), pp. 32330-32337
Abstract: Synemin is a large intermediate filament (IF) protein that has been identified in all types of muscle cells in association with desmin- and/or vimentin-containing IFs. Our previous studies (Bellin, R. M., Sernett, S. W., Becker, B., Ip, W., Huiatt, T. W., and Robson, R. M. (1999) J. Biol. Chem. 274, 29493-29499) demonstrated that synemin forms heteropolymeric IFs with major IF proteins and contains a binding site for the myofibrillar Z-line protein alpha-actinin. By utilizing blot overlay assays, we show herein that synemin also interacts with the costameric protein vinculin. Furthermore, extensive assays utilizing the Gal4 yeast two-hybrid system demonstrate interactions of synemin with desmin and vimentin and additionally define more precisely the protein subdomains involved in the synemin/alpha-actinin and synemin/vinculin interactions. The C-terminal approximately 300-amino acid region of synemin binds to the N-terminal head and central rod domains of alpha-actinin and the approximately 150-amino acid C-terminal tail of vinculin. Overall, these interactions indicate that synemin may anchor IFs to myofibrillar Z-lines via interactions with alpha-actinin and to costameres at the sarcolemma via interactions with vinculin and/or alpha-actinin. These linkages would enable the IFs to directly link all cellular myofibrils and to anchor the peripheral layer of myofibrils to the costameres.
DOI Link: 10.1074/jbc.M104005200
ISSN: 0021-9258
Links: http://hdl.handle.net/2381/14402
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Biochemistry

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