Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/14497
Full metadata record
DC FieldValueLanguage
dc.contributor.authorChew, Shern L.-
dc.contributor.authorBaginsky, Lysa-
dc.contributor.authorEperon, Ian C.-
dc.date.accessioned2012-10-24T08:58:14Z-
dc.date.available2012-10-24T08:58:14Z-
dc.date.issued2000-1-15-
dc.identifier.citationNucleic Acids Research, 2000, 28 (2), pp. 402-410-
dc.identifier.issn0305-1048-
dc.identifier.urihttp://hdl.handle.net/2381/14497-
dc.identifier.urihttp://nar.oxfordjournals.org/content/28/2/402-
dc.description.abstractAlternative pre-mRNA splicing of two terminal exons (α and β) regulates the expression of the human DNA ligase III gene. In most tissues, the α exon is expressed. In testes and during spermatogenesis, the β exon is used instead. The α exon encodes the interaction domain with a scaffold DNA repair protein, XRCC1, while the β exon-encoded C-terminal does not. Sequence elements regulating the alternative splicing pattern were mapped by in vitro splicing assays in HeLa nuclear extracts. Deletion of a region beginning in the β exon and extending into the downstream intron derepressed splicing to the β exon. Two silencing elements were found within this 101 nt region: a 16 nt exonic splicing silencer immediately upstream of the β exon polyadenylation signal and a 45 nt intronic splicing silencer. The exonic splicing silencer inhibited splicing, even when the poly­adenylation signal was deleted or replaced by a 5′ splice site. This element also enhanced polyadenylation under conditions unfavourable to splicing. The splicing silencer partially inhibited assembly of spliceo­somal complexes and functioned in an adenoviral pre-mRNA context. Silencing of splicing by the element was associated with cross-linking of a 37 kDa protein to the RNA substrate. The element exerts opposite functions in splicing and polyadenylation.-
dc.language.isoEnglish-
dc.publisherOxford University Press (OUP)-
dc.rightsArchived with reference to SHERPA/RoMEO and publisher website.-
dc.sourceWeb of Science-
dc.source.urihttp://webofknowledge.com-
dc.subjectScience & Technology-
dc.subjectLife Sciences & Biomedicine-
dc.subjectBiochemistry & Molecular Biology-
dc.subjectPRE-MESSENGER-RNA-
dc.subjectIMMUNODEFICIENCY-VIRUS TYPE-1-
dc.subjectFIBRONECTIN EDA EXON-
dc.subjectMEIOTIC RECOMBINATION-
dc.subjectMOLECULAR-CLONING-
dc.subjectPOLYADENYLATION-
dc.subjectPROTEINS-
dc.subjectGENE-
dc.subjectSPLICEOSOME-
dc.subjectINTRON-
dc.titleAn exonic splicing silencer in the testes-specific DNA ligase III beta exon-
dc.typeJournal Article-
dc.identifier.doi10.1093/nar/28.2.402-
dc.identifier.eissn1362-4962-
dc.description.irispid14109-
Appears in Collections:Published Articles, Dept. of Biochemistry

Files in This Item:
File Description SizeFormat 
Nucl. Acids Res.-2000-Chew-402-10.pdf868.77 kBAdobe PDFView/Open


Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.