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Title: Lineage tracing of cardiac explant derived cells.
Authors: Shenje, Lincoln T.
Field, L. J.
Pritchard, Catrin A.
Guerin, Christopher J.
Rubart, M.
Soonpaa, M. H.
Ang, Keng-Leong
Galiñanes, Manuel
First Published: 16-Apr-2008
Publisher: Public Library of Science
Citation: PLoS ONE, 2008, 3 (4), pp. e1929-e1929
Abstract: Aims Cultured cardiac explants produce a heterogeneous population of cells including a distinctive population of refractile cells described here as small round cardiac explant derived cells (EDCs). The aim of this study was to explore the source, morphology and cardiogenic potential of EDCs. Methods Transgenic MLC2v-Cre/ZEG, and actin-eGFP mice were used for lineage-tracing of EDCs in vitro and in vivo. C57B16 mice were used as cell transplant recipients of EDCs from transgenic hearts, as well as for the general characterisation of EDCs. The activation of cardiac-specific markers were analysed by: immunohistochemistry with bright field and immunofluorescent microscopy, electron microscopy, PCR and RT-PCR. Functional engraftment of transplanted cells was further investigated with calcium transient studies. Results Production of EDCs was highly dependent on the retention of blood-derived cells or factors in the cultured explants. These cells shared some characteristics of cardiac myocytes in vitro and survived engraftment in the adult heart in vivo. However, EDCs failed to differentiate into functional cardiac myocytes in vivo as demonstrated by the absence of stimulation-evoked intracellular calcium transients following transplantation into the peri-infarct zone. Conclusions This study highlights that positive identification based upon one parameter alone such as morphology or immunofluorescene is not adequate to identify the source, fate and function of adult cardiac explant derived cells.
DOI Link: 10.1371/journal.pone.0001929
ISSN: 1932-6203
eISSN: 1932-6203
Type: Journal Article
Rights: Copyright: 2008 Shenje et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Appears in Collections:Published Articles, Dept. of Biochemistry

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