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Title: Pneumolysin generates multiple conductance pores in the membrane of nucleated cells.
Authors: El-Rachkidy, RG
Davies, NW
Andrew, PW
First Published: 11-Apr-2008
Citation: BIOCHEM BIOPHYS RES COMMUN, 2008, 368 (3), pp. 786-792
Abstract: Patch clamping was used to investigate, for the first time, the electrophysiological properties of pores formed by a cholesterol-dependent cytolysin in the membrane of nucleated cells rather than in artificial membranes as previously. Pneumolysin pores in inside-out patches of CHO cell membranes had a broad range of conductance classified into small (<200 pS), medium (>200 pS and <1 nS) and large (>1 nS). A lytic-deficient mutant of pneumolysin (W433F) induced a similar spectrum of channels but there were more small channels than with wild-type (WT) toxin and the number of events was decreased. The WT toxin also induced channels when given to the inside surface of the plasma membrane.
DOI Link: 10.1016/j.bbrc.2008.01.151
eISSN: 1090-2104
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Cell Physiology and Pharmacology

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