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|Title:||Evaluation of molecular-Beacon, TaqMan, and fluorescence resonance energy transfer probes for detection of antibiotic resistance-conferring single nucleotide polymorphisms in mixed Mycobacterium tuberculosis DNA extracts.|
LONG DRUG Study Group
|Citation:||J CLIN MICROBIOL, 2006, 44 (10), pp. 3826-3829|
|Abstract:||The ability of fluorescence resonance energy transfer, molecular-beacon, and TaqMan probes to detect single nucleotide polymorphism (SNP) in the presence of a wild-type allele was evaluated using drug resistance-conferring SNPs in mixed Mycobacterium tuberculosis DNA. It was found that both the absolute quantity and the ratio of alleles determine the detection sensitivity of the probe systems.|
|Appears in Collections:||Published Articles, Dept. of Infection, Immunity and Inflammation|
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