Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/16137
Title: Quantitation of trans-resveratrol and detection of its metabolites in human plasma and urine by high performance liquid chromatography.
Authors: Boocock, DJ
Patel, KR
Faust, GE
Normolle, DP
Marczylo, TH
Crowell, JA
Brenner, DE
Booth, TD
Gescher, A
Steward, WP
First Published: 1-Apr-2007
Citation: J CHROMATOGR B ANALYT TECHNOL BIOMED LIFE SCI, 2007, 848 (2), pp. 182-187
Abstract: We describe a reversed-phase HPLC method that uses gradient elution and UV detection (325 nm) to determine levels of resveratrol and identify six major conjugated metabolites in the plasma and urine of human volunteers after administration of a single oral dose of 1g. Waters Atlantis C18 3 microm served as the stationary phase. The gradient was formed using ammonium acetate and methanol, containing 2% propan-2-ol. Detection is linear between 5 ng/mL and 500 ng/mL in plasma (5-1000 ng/mL in urine). The coefficient of variation for intra- and inter-day variation is <10%. The average recovery of resveratrol from plasma and urine is 58+/-3%. The data presented in this report demonstrate a rapid, sensitive and accurate method for the analysis of resveratrol and its metabolites in human plasma and urine for pharmacokinetic studies.
DOI Link: 10.1016/j.jchromb.2006.10.017
ISSN: 1570-0232
Links: http://hdl.handle.net/2381/16137
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Cancer Studies and Molecular Medicine

Files in This Item:
There are no files associated with this item.


Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.