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Title: The importance of careful blood processing in isolation of cell-free DNA.
Authors: Page, K
Powles, T
Slade, MJ
DE Bella MT
Walker, RA
Coombes, RC
Shaw, JA
First Published: Sep-2006
Citation: ANN N Y ACAD SCI, 2006, 1075, pp. 313-317
Abstract: In healthy individuals, the source of cell-free plasma DNA is predominantly apoptotic, whereas, increased plasma DNA integrity is seen in cancer patients. Therefore, it is important to carefully isolate absolutely "cell-free" plasma DNA. Plasma DNA from 30 healthy females was analyzed using 4 PCR amplicons of increasing size, comparing standard blood processing with additional centrifugation steps prior to DNA extraction. Cellular DNA contamination, indicated by positive amplicons >300 bp was eliminated only after the extra centrifugation step. This highlights the importance of careful processing in preparation of cell-free plasma DNA as a tool for cancer detection and we recommend the use of a microcentrifuge spin, prior to DNA extraction.
DOI Link: 10.1196/annals.1368.042
ISSN: 0077-8923
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Cancer Studies and Molecular Medicine

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