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Title: Binding of GTPgamma[35S] is regulated by GDP and receptor activation. Studies with the nociceptin/orphanin FQ receptor.
Authors: McDonald, J
Lambert, DG
First Published: Mar-2010
Citation: BR J PHARMACOL, 2010, 159 (6), pp. 1286-1293
Abstract: We have examined the effects of ligand efficacy and receptor density on the binding of guanosine 5'-[gamma-thio]triphosphate (GTPgammaS) and GDP to the nociceptin/orphanin FQ (N/OFQ) peptide receptor (NOP)-coupled G-proteins. Experimental approach: In GTPgamma[(35)S] binding experiments, using stable (CHO(hNOP)) and inducible (CHO(INDhNOP)) recombinant human and rat NOP we have measured: (i) ligand-specific GDP requirements; (ii) the effects of receptor density on guanine nucleotide affinity/capacity; and (iii) the effect of ligand efficacy on GTPgammaS association kinetics.
DOI Link: 10.1111/j.1476-5381.2009.00621.x
eISSN: 1476-5381
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Cardiovascular Sciences

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