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|Title:||Kv1.3 is the exclusive voltage-gated K+ channel of platelets and megakaryocytes: roles in membrane potential, Ca2+ signalling and platelet count.|
Snowden, Roger T.
Kaczmarek, L. K.
Goodall, Alison H.
Forsythe, Ian D.
Mahaut-Smith, Martyn P.
|Citation:||The Journal of Physiology, 2010, 588 (Pt 9), pp. 1399-1406|
|Abstract:||A delayed rectifier voltage-gated K(+) channel (Kv) represents the largest ionic conductance of platelets and megakaryocytes, but is undefined at the molecular level. Quantitative RT-PCR of all known Kv alpha and ancillary subunits showed that only Kv1.3 (KCNA3) is substantially expressed in human platelets. Furthermore, megakaryocytes from Kv1.3(/) mice or from wild-type mice exposed to the Kv1.3 blocker margatoxin completely lacked Kv currents and displayed substantially depolarised resting membrane potentials. In human platelets, margatoxin reduced the P2X(1)- and thromboxaneA(2) receptor-evoked [Ca(2+)](i) increases and delayed the onset of store-operated Ca(2+) influx. Megakaryocyte development was normal in Kv1.3(/) mice, but the platelet count was increased, consistent with a role of Kv1.3 in apoptosis or decreased platelet activation. We conclude that Kv1.3 forms the Kv channel of the platelet and megakaryocyte, which sets the resting membrane potential, regulates agonist-evoked Ca(2+) increases and influences circulating platelet numbers.|
|Rights:||Copyright © 2010 The Authors. Journal compilation © 2010 The Physiological Society. This is a Wiley OnlineOpen article, used in accordance with the terms at http://olabout.wiley.com/WileyCDA/Section/id-406241.html#OnlineOpen_Terms|
|Appears in Collections:||Published Articles, Dept. of Cell Physiology and Pharmacology|
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