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|Title:||A YY1-binding site is required for accurate human LINE-1 transcription initiation.|
|Authors:||Athanikar, J .N.|
Badge, Richard M.
Moran, J. V.
|Publisher:||Oxford University Press (OUP)|
|Citation:||Nucleic Acids Research, 2004, 32 (13), pp.3846-3855|
|Abstract:||The initial step in Long Interspersed Element-1 (LINE-1) retrotransposition requires transcription from an internal promoter located within its 5′-untranslated region (5′-UTR). Previous studies have identified a YY1 (Yin Yang 1)-binding site as an important sequence in LINE-1 transcription. Here, we demonstrate that mutations in the YY1-binding site have only minor effects on transcription activation of the full-length 5′-UTR and LINE-1 mobility in a single round cultured cell retrotransposition assay. Instead, these mutations disrupt proper initiation of transcription from the +1 site of the 5′-UTR. Thus, we propose that the YY1-binding site functions as a component of the LINE-1 core promoter to direct accurate transcription initiation. Indeed, this sequence may explain the evolutionary success of LINE-1 by enabling full-length retrotransposed copies to undergo autonomous retrotransposition in subsequent generations.|
|Rights:||Archived with reference to SHERPA/RoMEO and publisher website.|
|Appears in Collections:||Published Articles, Dept. of Genetics|
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