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|Title:||DASH-2: flexible, low-cost, and high-throughput SNP genotyping by dynamic allele-specific hybridization on membrane arrays|
Howell, W. M.
Brookes, Anthony J.
|Publisher:||Cold Spring Harbor Laboratory Press|
|Citation:||Genome Research, 2003, 13 (5), pp.916-924|
|Abstract:||Genotyping technologies need to be continually improved in terms of their flexibility, cost-efficiency, and throughput, to push forward genome variation analysis. To this end, we have leveraged the inherent simplicity of dynamic allele-specific hybridization (DASH) and coupled it to recent innovations of centrifugal arrays and iFRET. We have thereby created a new genotyping platform we term DASH-2, which we demonstrate and evaluate in this report. The system is highly flexible in many ways (any plate format, PCR multiplexing, serial and parallel array processing, spectral-multiplexing of hybridization probes), thus supporting a wide range of application scales and objectives. Precision is demonstrated to be in the range 99.8–100%, and assay costs are 0.05 USD or less per genotype assignment. DASH-2 thus provides a powerful new alternative for genotyping practice, which can be used without the need for expensive robotics support.|
|Rights:||Copyright © 2003 by Cold Spring Harbor Laboratory Press Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/).|
|Appears in Collections:||Published Articles, Dept. of Genetics|
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|Genome Res.-2003-Jobs-916-24.pdf||Publisher version||915.49 kB||Adobe PDF||View/Open|
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