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Title: DASH-2: flexible, low-cost, and high-throughput SNP genotyping by dynamic allele-specific hybridization on membrane arrays
Authors: Jobs, M.
Howell, W. M.
Strömqvist, L.
Mayr, T.
Brookes, Anthony J.
First Published: 2003
Publisher: Cold Spring Harbor Laboratory Press
Citation: Genome Research, 2003, 13 (5), pp.916-924
Abstract: Genotyping technologies need to be continually improved in terms of their flexibility, cost-efficiency, and throughput, to push forward genome variation analysis. To this end, we have leveraged the inherent simplicity of dynamic allele-specific hybridization (DASH) and coupled it to recent innovations of centrifugal arrays and iFRET. We have thereby created a new genotyping platform we term DASH-2, which we demonstrate and evaluate in this report. The system is highly flexible in many ways (any plate format, PCR multiplexing, serial and parallel array processing, spectral-multiplexing of hybridization probes), thus supporting a wide range of application scales and objectives. Precision is demonstrated to be in the range 99.8–100%, and assay costs are 0.05 USD or less per genotype assignment. DASH-2 thus provides a powerful new alternative for genotyping practice, which can be used without the need for expensive robotics support.
DOI Link: 10.1101/gr.801103
ISSN: 1088-9051
Type: Article
Rights: Copyright © 2003 by Cold Spring Harbor Laboratory Press Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License,
Appears in Collections:Published Articles, Dept. of Genetics

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