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Title: Novel immunogenic peptides elicit systemic anaphylaxis in mice: implications for peptide vaccines.
Authors: Smith, CM
Bradding, P
Neill, DR
Baxendale, H
Felici, F
Andrew, PW
First Published: 1-Aug-2011
Citation: J IMMUNOL, 2011, 187 (3), pp. 1201-1206
Abstract: Peptide-based therapies are showing increasing potential for the development of vaccines and in the treatment of many important diseases. We previously reported two peptide conjugate vaccines that protected mice against pneumococcal disease. During this study, we observed an unexpected phenomenon; several vaccine candidates induced a rapid, fatal anaphylaxis after booster injection of the peptide conjugate. Further investigation indicated the reaction was mediated by the production of peptide-specific IgE and the release of histamine. Notably, among seven peptides tested, all of which bound the same mAb that selected them from a phage library, only four elicited this severe reaction. Sequence alignment analysis of all peptides revealed unique clusters of acidic amino acid residues in the allergenic peptides. Substitution of the acidic amino acid residues, ED, of peptide MP2 with their amine equivalents, QN, eliminated the anaphylactic effects but did not affect the production of peptide-specific IgG. These results have important implications for both the study of allergens and the development of future peptide-based therapies.
DOI Link: 10.4049/jimmunol.1002152
eISSN: 1550-6606
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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