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Title: Protein binding interactions at the STB locus of the yeast 2μm plasmid
Authors: Hadfield, Christopher
Mount, Robert C.
Cashmore, Annette M.
First Published: 25-Mar-1995
Publisher: Oxford University Press
Citation: Nucleic Acids Research, 1995, 23 (6), pp. 995-1002
Abstract: The cis-acting STB locus has been shown to be a multiple protein binding site. STB-specific binding activity was detected in a normally insoluble yeast cell protein fraction, suggesting association with a sub-cellular structure. Both 2pm-encoded and host-encoded STB-binding activities were identified. The 2μm proteins showed contrasting STB-binding activities: C (REP2) protein acted cooperatively with the host factor to promote STB binding; B (REP1) protein also acted in association with the host factor, but showed a dual action, opposing or facilitating binding, depending upon concentration; D (RAF) exhibited rapid binding and antagonism to host factor binding. FLP did not bind, but promoted host factor dissociation. The implications of these activities for the molecular mechanism of 2μm plasmid inheritance are considered.
DOI Link: 10.1093/nar/23.6.995
ISSN: 0305-1048
Type: Journal Article
Rights: Copyright © 1995 Oxford University Press
Appears in Collections:Published Articles, Dept. of Genetics

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