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Title: Minisatellite isoalleles can be distinguished by single-stranded conformational polymorphism analysis in agarose gels
Authors: Monckton, Darren G.
Jeffreys, Alec J.
First Published: 11-Jun-1994
Publisher: Oxford University Press
Citation: Nucleic Acids Research, 1994, 22 (11), pp. 2155-2157
Abstract: Minisatellite isoallelism, i.e. the occurrence of minisatellite alleles with different internal sequence composition but indistinguishable length, is a common limitation of minisatellite allele length analysis. Internal sequence variation can be used to distinguish such isoalleles, provided that detailed sequence knowledge of its basis is available. We now show that minisatellite isoalleles can also be simply resolved by single-stranded conformational polymorphisms (SSCP) arising during agarose gel electrophoresis. SSCP on agarose gels can be used to distinguish minisatellite isoalleles either after PCR amplification, or by standard Southern blot analysis of genomic DNA.
DOI Link: 10.1093/nar/22.11.2155
ISSN: 0305-1048
Type: Journal Article
Rights: Copyright © Oxford University Press 1994
Appears in Collections:Published Articles, Dept. of Genetics

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