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Authors: Collick, A.
Dunn, M. G.
Jeffreys, A. J.
First Published: 11-Dec-1991
Publisher: Oxford University Press (OUP)
Citation: Nucleic Acids Research, 1991, 19 (23), pp. 6399-6404
Abstract: Msbp-1 is a minisatellite-specific DNA-binding protein. Using synthetic binding substrates, we now show that Msbp-1 binds not to double-stranded DNA, but exclusively to single-stranded DNA. Binding is specific to the guanine-rich strand of the minisatellite duplex, interactions with the cytosine-rich strand being undetectable by southwestern analysis. Furthermore, the binding site required for successful DNA-protein interactions appears to be two or more minisatellite repeat units. We have also isolated, by whole-genome PCR and cloning, one Msbp-1 binding site from the human genome. Again, the binding strand of this molecule contains a repetitive G-rich structure equivalent to that of a small minisatellite. These observations are discussed with respect to other single-stranded DNA-binding proteins known to play a role in recombination processes.
DOI Link: 10.1093/nar/19.23.6399
ISSN: 0305-1048
Type: Journal Article
Rights: Copyright © 1991 Oxford University Press
Appears in Collections:Published Articles, Dept. of Genetics

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