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|Title:||MINISATELLITE BINDING-PROTEIN MSBP-1 IS A SEQUENCE-SPECIFIC SINGLE-STRANDED DNA-BINDING PROTEIN|
Dunn, M. G.
Jeffreys, A. J.
|Publisher:||Oxford University Press (OUP)|
|Citation:||Nucleic Acids Research, 1991, 19 (23), pp. 6399-6404|
|Abstract:||Msbp-1 is a minisatellite-specific DNA-binding protein. Using synthetic binding substrates, we now show that Msbp-1 binds not to double-stranded DNA, but exclusively to single-stranded DNA. Binding is specific to the guanine-rich strand of the minisatellite duplex, interactions with the cytosine-rich strand being undetectable by southwestern analysis. Furthermore, the binding site required for successful DNA-protein interactions appears to be two or more minisatellite repeat units. We have also isolated, by whole-genome PCR and cloning, one Msbp-1 binding site from the human genome. Again, the binding strand of this molecule contains a repetitive G-rich structure equivalent to that of a small minisatellite. These observations are discussed with respect to other single-stranded DNA-binding proteins known to play a role in recombination processes.|
|Rights:||Copyright © 1991 Oxford University Press|
|Appears in Collections:||Published Articles, Dept. of Genetics|
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