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Title: Construction of a chromosome-enriched Hpall library from flow-sorted wheat chromosomes
Authors: Wang, M. L.
Leitch, A. R.
Schwarzacher, T.
Heslopharrison, J. S.
Moore, G.
First Published: 25-Apr-1992
Publisher: Oxford University Press
Citation: Nucleic Acids Research, 1992, 20 (8), pp. 1897-1901
Abstract: We report here the first successful generation of a chromosome-enriched library from flow sorted plant chromosomes. Chromosomes with a characteristic DNA content (a peak) were sorted from a synchronized cell culture (TaKB1, derived from Triticum aestlvum). A Hpall library was constructed from the sorted chromosomes and half of the cloned DNA sequences analysed are unique or low copy. Approximately half of these sequences when used as probes detect sequences on wheat chromosome 4A. The generation and analysis of the chromosome library is described in detail and the prospects of using flow-sorted plant chromosomes discussed.
DOI Link: 10.1093/nar/20.8.1897
ISSN: 0305-1048
Type: Journal Article
Rights: Copyright © 1992 Oxford University Press
Appears in Collections:Published Articles, Dept. of Biology

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