Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/23171
Title: Immunocytochemical identification of G-protein-coupled receptor expression and localization.
Authors: Mongan, LC
Grubb, BD
First Published: 2004
Citation: METHODS MOL BIOL, 2004, 259, pp. 67-80
Abstract: Immunocytochemistry exploits the incomparable specificity of the antibody-antigen interaction to form the basis of a flexible approach to the study of expression and localization of proteins both in model systems and their physiological context. This chapter details the theory and practice of the technique as well as lists the materials required. A general protocol is proposed, which can be adapted to suit the needs of individual investigators using the suggestions outlined in the Notes. The use of frozen tissue sections and cultured cells is described. Finally, the most common causes for failure of the technique are presented, along with likely solutions.
DOI Link: 10.1385/1-59259-754-8:067
ISSN: 1064-3745
Links: http://hdl.handle.net/2381/23171
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Cell Physiology and Pharmacology

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