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Title: A streamlined method for systematic, high resolution in situ analysis of mRNA distribution in plants.
Authors: Drea, S
Corsar, J
Crawford, B
Shaw, P
Dolan, L
Doonan, JH
First Published: 2005
Citation: PLANT METHODS, 2005, 1 (1), pp. 8-8
Abstract: In situ hybridisation can provide cellular, and in some cases sub-cellular, resolution of mRNA levels within multicellular organisms and is widely used to provide spatial and temporal information on gene expression. However, standard protocols are complex and laborious to implement, restricting analysis to one or a few genes at any one time. Whole-mount and reverse transcriptase-PCR (RT-PCR) based protocols increase throughput, but can compromise both specificity and resolution. With the advent of genome-wide analysis of gene expression, there is an urgent need to develop high-throughput in situ methods that also provide high resolution.
DOI Link: 10.1186/1746-4811-1-8
eISSN: 1746-4811
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Biology

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