Please use this identifier to cite or link to this item:
Title: Proliferation and cell fate establishment during Arabidopsis male gametogenesis depends on the Retinoblastoma protein.
Authors: Chen, Z
Hafidh, S
Poh, SH
Twell, D
Berger, F
First Published: 28-Apr-2009
Citation: PROC NATL ACAD SCI U S A, 2009, 106 (17), pp. 7257-7262
Abstract: The Retinoblastoma (Rb) protein is a conserved repressor of cell proliferation. In animals and plants, deregulation of Rb protein causes hyperproliferation and perturbs cell differentiation to various degrees. However, the primary developmental impact of the loss of Rb protein has remained unclear. In this study we investigated the direct consequences of Rb protein knockout in the Arabidopsis male germline using cytological and molecular markers. The Arabidopsis germ line derives from the unequal division of the microspore, producing a small germ cell and a large terminally differentiated vegetative cell. A single division of the germ cell produces the 2 sperm cells. We observed that the loss of Rb protein does not have a major impact on microspore division but causes limited hyperproliferation of the vegetative cell and, to a lesser degree, of the sperm cells. In addition, cell fate is perturbed in a fraction of Rb-defective vegetative cells. These defects are rescued by preventing cell proliferation arising from down-regulation of cyclin-dependent kinase A1. Our results indicate that hyperproliferation caused by the loss of Rb protein prevents or delays cell determination during plant male gametogenesis, providing further evidence for a direct link between fate determination and cell proliferation.
DOI Link: 10.1073/pnas.0810992106
eISSN: 1091-6490
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Biology

Files in This Item:
There are no files associated with this item.

Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.