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Title: A 15n-filtered 2D 1H TOCSY experiment for assignment of aromatic ring resonances and selective identification of tyrosine ring resonances in proteins: Description and application to Photoactive Yellow Protein.
Authors: Whitehead, B
Tessari, M
Düx, P
Boelens, R
Kaptein, R
Vuister, GW
First Published: Apr-1997
Citation: J BIOMOL NMR, 1997, 9 (3), pp. 313-316
Abstract: A new method to selectively detect the ring resonances of the aromatic residues in 15N-labelled proteins is presented. The experiment consists of a 2D 1H TOCSY sequence withremoval of the amide signals via 15N-filtering. Experiments are acquired in the absence andpresence of water inversion; combining the two spectra allows selective observation of thetyrosine ring resonances and enables the identification of their delta andepsilon ring protons. The experiment is demonstrated on a 15N-labelled sample of Photoactive Yellow Protein and isshown to give good selectivity for tyrosine ring resonances under a wide range oftemperatures and pH values.
DOI Link: 10.1023/A:1018687127330
ISSN: 0925-2738
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Biochemistry

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