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|Title:||Studying Arabidopsis chloroplast structural organisation using transmission electron microscopy.|
|Citation:||METHODS MOL BIOL, 2011, 774, pp. 113-132|
|Abstract:||Chloroplasts, as well as other, non-photosynthetic types of plastid, are characteristic structures within plant cells. They are relatively large organelles (typically 1-5 μm in diameter), and so can readily be analysed by electron microscopy. Chloroplast structure is remarkably complex, comprising at least six distinct sub-organellar compartments, and is sensitive to developmental changes, environmental effects, and genetic lesions. Transmission electron microscopy (TEM), therefore, represents a powerful technique for monitoring the effects of various changing parameters or treatments on the development and differentiation of these important organelles. We describe a method for the analysis of Arabidopsis plant material by TEM, primarily for the assessment of plastid ultrastructure.|
|Appears in Collections:||Published Articles, Dept. of Biology|
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