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Title: Conceptual translation of timeless reveals alternative initiating methionines in Drosophila
Authors: Rosato, Ezio
Kyriacou, Charalambos P.
Trevisan, A.
Sandrelli, F.
Zordan, M.
Costa, R.
First Published: 1997
Publisher: Oxford University Press (OUP)
Citation: Nucleic Acids Research, 1997, 25 (3), pp. 455-457
Abstract: We have sequenced genomic fragments which encode the N-terminus of the TIMELESS (TIM) clock protein in Drosophila simulans and D.yakuba. We observe that in these two species, the initiating methionine appears to lie downstream of the one proposed to encode the translational start in D.melanogaster, thereby truncating the N-terminus by 23 amino acids. We then sequenced the corresponding 5′ fragment in a number of D.melanogaster individuals from different strains. We observed a polymorphism which strongly suggests that the originally proposed start site cannot be utilised in some individuals, and that these flies will initiate translation of TIM at the downstream ATG. Given the current interest in TIM regulation in D.melanogaster, it is important to correctly define the N-terminus in this species.
DOI Link: 10.1093/nar/25.3.455
ISSN: 0305-1048
eISSN: 1362-4962
Type: Journal Article
Rights: Archived with reference to SHERPA/RoMEO and publisher website. Copyright 1997 Oxford University Press. Version of record:
Description: DDBJ/EMBL/GenBank accession nos Y09930–Y09937
Appears in Collections:Published Articles, Dept. of Genetics

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