Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/24162
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dc.contributor.authorBayliss, C. D.-
dc.contributor.authorSmith, G. L.-
dc.date.accessioned2012-10-24T09:14:19Z-
dc.date.available2012-10-24T09:14:19Z-
dc.date.issued1997-10-15-
dc.identifier.citationNucleic Acids Research, 1997, 25 (20), pp. 3984-3990-
dc.identifier.issn0305-1048-
dc.identifier.urihttp://hdl.handle.net/2381/24162-
dc.identifier.urihttp://nar.oxfordjournals.org/content/25/20/3984-
dc.description.abstractVaccinia virus protein VP8 is a 25 kDa product of the L4R gene and is an abundant virion protein that binds single-stranded (ss) and double-stranded (ds) DNA. Binding of ssDNA is preferred at high salt concentrations. Using a recombinant 25 kDa L4R (rL4R) protein and a gel mobility shift assay with radiolabelled oligonucleotides, the K[subscript: d] for a 45mer oligonucleotide was determined to be 2 nM. The K[subscript: d] was unaltered by 50 mM KCl but was reduced 35-fold by 100 mM KCl. Multiple rL4R molecules bound to a single 45mer oligonucleotide, and using oligonucleotides of different lengths it was calculated that one rL4R molecule bound every 17 nt. Binding to ssDNA was competed by both deoxyribo- and ribopolynucleotides. RNA binding was observed for both rL4R and native VP8, purified from virions, using a gel mobility shift with a radiolabelled ssRNA of 130 nt. The K[subscript: d] of rL4R for this ssRNA substrate was 3 nM in the absence of salt and binding was positively cooperative. The potential roles of L4R protein in vaccinia virus early transcription are discussed.-
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dc.rightsCopyright © 1997 Oxford University Press-
dc.sourceScopus-
dc.source.urihttp://www.scopus.com/home.url-
dc.titleVaccinia virion protein VP8, the 25 kDa product of the L4R gene, binds single-stranded DNA and RNA with similar affinity-
dc.typeJournal Article-
dc.identifier.doi10.1093/nar/25.20.3984-
dc.description.irispid77285-
Appears in Collections:Published Articles, Dept. of Genetics

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