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|dc.contributor.author||Bayliss, C. D.||-|
|dc.contributor.author||Smith, G. L.||-|
|dc.identifier.citation||Nucleic Acids Research, 1997, 25 (20), pp. 3984-3990||-|
|dc.description.abstract||Vaccinia virus protein VP8 is a 25 kDa product of the L4R gene and is an abundant virion protein that binds single-stranded (ss) and double-stranded (ds) DNA. Binding of ssDNA is preferred at high salt concentrations. Using a recombinant 25 kDa L4R (rL4R) protein and a gel mobility shift assay with radiolabelled oligonucleotides, the K[subscript: d] for a 45mer oligonucleotide was determined to be 2 nM. The K[subscript: d] was unaltered by 50 mM KCl but was reduced 35-fold by 100 mM KCl. Multiple rL4R molecules bound to a single 45mer oligonucleotide, and using oligonucleotides of different lengths it was calculated that one rL4R molecule bound every 17 nt. Binding to ssDNA was competed by both deoxyribo- and ribopolynucleotides. RNA binding was observed for both rL4R and native VP8, purified from virions, using a gel mobility shift with a radiolabelled ssRNA of 130 nt. The K[subscript: d] of rL4R for this ssRNA substrate was 3 nM in the absence of salt and binding was positively cooperative. The potential roles of L4R protein in vaccinia virus early transcription are discussed.||-|
|dc.rights||Copyright © 1997 Oxford University Press||-|
|dc.title||Vaccinia virion protein VP8, the 25 kDa product of the L4R gene, binds single-stranded DNA and RNA with similar affinity||-|
|Appears in Collections:||Published Articles, Dept. of Genetics|
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