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Title: Determination of quercetin in human plasma by HPLC with spectrophotometric or electrochemical detection.
Authors: Jones, DJ
Lim, CK
Ferry, DR
Gescher, A
First Published: Jul-1998
Citation: BIOMED CHROMATOGR, 1998, 12 (4), pp. 232-235
Abstract: A reversed-phase high-performance liquid chromatographic method for the determination of quercetin in human plasma following intravenous infusion is described. Quercetin in plasma was extracted with methanol-dimethyl sulphoxide (4:1 v/v) and separated on a C18 Hypersil-BDS column with 44% (v/v) methanol in 0.1 M ammonium acetate (pH 5.15) containing 0.27 mM EDTA as the mobile phase. The drug was detected specifically and sensitively at its absorption maximum of 375 nm, or electrochemically, with a detection limit of 80 ng/mL and 2 ng/mL, respectively.
DOI Link: 10.1002/(SICI)1099-0801(199807/08)12:4<232::AID-BMC740>3.0.CO;2-1
ISSN: 0269-3879
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Cancer Studies and Molecular Medicine

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