Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/25648
Title: The Hybrid Origin of Two Cultivars of Crocus (Iridaceae) Analysed by Molecular Cytogenetics including Genomic Southern and in situ Hybridization
Authors: Ørgaard, M.
Jacobsen, N.
Heslop-Harrison, J. S.
First Published: Sep-1995
Citation: Annals of Botany, 1995, 76 (3), pp. 253-262
Abstract: The origin of the two common cultivars of Crocus, C. 'Stellaris' (2n = 2x = 10) and C. 'Golden Yellow' (2n = 3x = 14) was investigated by fluorescent in situ hybridization using both total genomic DNA and cloned DNA sequences as probes. The clear differentiation between the chromosomes after genomic in situ hybridization supports the proposals of a hybrid origin of the cultivars and shows that they have the same parental genomes originating from C. flavus (2n = 8) and C. angustifolius (2n = 12). C. 'Stellaris' has four chromosomes of C. flavus origin and six chromosomes of C. angustifolius origin. C. 'Golden Yellow' has eight chromosomes of C. flavus origin and six chromosomes of C. angustifolius origin. The number and location of 18S-5·8S-26S rRNA genes on the chromosomes of the hybrids and of the parental species agree with the results from the genomic probings. Hybridization to Southern membranes also supports the hybrid origin of C. 'Golden Yellow'.
DOI Link: 10.1006/anbo.1995.1094
ISSN: 0305-7364
eISSN: 1095-8290
Links: http://hdl.handle.net/2381/25648
http://aob.oxfordjournals.org/content/76/3/253
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Biology

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