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Title: High performance liquid chromatography of tamoxifen and metabolites in plasma and tissues.
Authors: Lim, CK
Chow, LC
Yuan, ZX
Smith, LL
First Published: Nov-1993
Citation: BIOMED CHROMATOGR, 1993, 7 (6), pp. 311-314
Abstract: An isocratic reversed-phase high performance liquid chromatographic method for the determination of tamoxifen and its metabolites in plasma and tissues is described. Plasma or tissue homogenate was extracted with methanol/dimethyl sulphoxide (4:1 v/v). The supernatant after centrifugation was separated on a BDS-Hypersil column with methanol/0.5 M ammonium acetate (75:25 v/v) as the mobile phase. The recoveries of tamoxifen added to plasma and liver tissue homogenate by the extraction procedure were 102 +/- 1.6 and 98 +/- 2.4% (mean +/- SD, n = 6), respectively. The solutes were detected at 280 nm with a detection limit of 0.25 micrograms/mL for tamoxifen.
DOI Link: 10.1002/bmc.1130070606
ISSN: 0269-3879
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Biochemistry

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