Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/26540
Title: Spontaneous mutation rates to new length alleles at tandem-repetitive hypervariable loci in human DNA.
Authors: Jeffreys, AJ
Royle, NJ
Wilson, V
Wong, Z
First Published: 17-Mar-1988
Citation: NATURE, 1988, 332 (6161), pp. 278-281
Abstract: Tandem-repetitive minisatellite regions in vertebrate DNA frequently show substantial allelic variation in the number of repeat units. This variation is thought to arise through processes such as unequal crossover or replication slippage. We show here that the spontaneous mutation rate to new length alleles at extremely variable human minisatellites is sufficiently high to be directly measurable in human pedigrees. The mutation rate at different loci increases with variability in accord with the neutral mutation/random drift hypothesis, and rises to 5% per gamete for the most unstable human minisatellite isolated. Mutations are sporadic, occur with similar frequencies in sperm and oocytes, and can involve the gain or loss of substantial numbers of repeat units, consistent with length changes arising primarily by unequal exchange at meiosis. Germline instability must therefore be taken into account when using hypervariable loci as genetic markers, particularly in pedigree analysis and parenthood testing.
DOI Link: 10.1038/332278a0
ISSN: 0028-0836
Links: http://hdl.handle.net/2381/26540
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Genetics

Files in This Item:
There are no files associated with this item.


Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.