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Title: Synthetic ligands for apo-neocarzinostatin.
Authors: Caddick, S
Muskett, FW
Stoneman, RG
Woolfson, DN
First Published: 5-Apr-2006
Citation: J AM CHEM SOC, 2006, 128 (13), pp. 4204-4205
Abstract: Neocarzinostatin (NCS) is a 1:1 complex of an enediyne chromophore (NCSChrom), non-covalently bound to an 11 kDa protein (apoNCS). We are exploring apoNCS as a generic protein system for sequestering small molecules for therapeutic applications. Here, we disclose a new flavone ligand 1 for apoNCS and present a high-resolution NMR structure of this ligand bound to apoNCS. This is the first high-resolution structure of a completely non-cognate ligand bound to the apoNCS protein. This work provides unambiguous evidence that a completely new class of ligand can bind specifically to apoNCS. Furthermore, the mode of binding is different than that of the naphthoate-based ligands, and for such a simple hydrophobic compound, the new ligand surprisingly binds specifically. This work indicates that apo-Neocarzinostatin has multiple selective and distinct binding modes for small-molecule cargo.
DOI Link: 10.1021/ja058419i
ISSN: 0002-7863
Type: Journal Article
Appears in Collections:Published Articles, Dept. of Biochemistry

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