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Title: Complexin-I Is Required for High-Fidelity Transmission at the Endbulb of Held Auditory Synapse
Authors: Strenzke, Nicola
Chanda, Soham
Kopp-Scheinpflug, Cornelia
Khimich, Darina
Reim, Kerstin
Bulankina, Anna V.
Neef, Andreas
Wolf, Fred
Brose, Nils
Xu-Friedman, Matthew A.
Moser, Tobias
First Published: 24-Jun-2009
Publisher: Society for Neuroscience
Citation: Journal of Neuroscience, 2009, 29 (25), pp. 7991-8004.
Abstract: Complexins (CPXs I–IV) presumably act as regulators of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex, but their function in the intact mammalian nervous system is not well established. Here, we explored the role of CPXs in the mouse auditory system. Hearing was impaired in CPX I knock-out mice but normal in knock-out mice for CPXs II, III, IV, and III/IV as measured by auditory brainstem responses. Complexins were not detectable in cochlear hair cells but CPX I was expressed in spiral ganglion neurons (SGNs) that give rise to the auditory nerve. Ca2+-dependent exocytosis of inner hair cells and sound encoding by SGNs were unaffected in CPX I knock-out mice. In the absence of CPX I, the resting release probability in the endbulb of Held synapses of the auditory nerve fibers with bushy cells in the cochlear nucleus was reduced. As predicted by computational modeling, bushy cells had decreased spike rates at sound onset as well as longer and more variable first spike latencies explaining the abnormal auditory brainstem responses. In addition, we found synaptic transmission to outlast the stimulus at many endbulb of Held synapses in vitro and in vivo, suggesting impaired synchronization of release to stimulus offset. Although sound encoding in the cochlea proceeds in the absence of complexins, CPX I is required for faithful processing of sound onset and offset in the cochlear nucleus.
DOI Link: 10.1523/JNEUROSCI.0632-09.2009
ISSN: 0270-6474
eISSN: 1529-2401
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2009 Society for Neuroscience. Deposited with reference to the publisher's archiving policy available on the SHERPA/RoMEO website. 6 months after publication, the work becomes available to the public to copy, distribute, or display under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license.
Appears in Collections:Published Articles, MRC Toxicology Unit

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