Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/28103
Title: Mycobacterium tuberculosis RNA Polymerase-binding Protein A (RbpA) and Its Interactions with Sigma Factors
Authors: Bortoluzzi, Alessio
Muskett, Frederick W.
Waters, Lorna C.
Addis, Philip W.
Rieck, Barbara
Munder, Thomas
Schleier, Susanne
Forti, Francesca
Ghisotti, Daniela
Carr, Mark D.
O'Hare, Helen M.
First Published: 22-Apr-2013
Publisher: American Society for Biochemistry and Molecular Biology
Citation: The Journal of Biological Chemistry, 2013, 288 (20), pp. 14438-14450
Abstract: RNA polymerase-binding protein A (RbpA), encoded by Rv2050, is specific to the actinomycetes, where it is highly conserved. In the pathogen Mycobacterium tuberculosis, RbpA is essential for growth and survival. RbpA binds to the β subunit of the RNA polymerase where it activates transcription by unknown mechanisms, and it may also influence the response of M. tuberculosis to the current frontline anti-tuberculosis drug rifampicin. Here we report the solution structure of RbpA and identify the principle sigma factor σ[superscript A] and the stress-induced σ[superscript B] as interaction partners. The protein has a central ordered domain with a conserved hydrophobic surface that may be a potential protein interaction site. The N and C termini are highly dynamic and are involved in the interaction with the sigma factors. RbpA forms a tight complex with the N-terminal domain of σB via its N- and C-terminal regions. The interaction with sigma factors may explain how RbpA stabilizes sigma subunit binding to the core RNA polymerase and thereby promotes initiation complex formation. RbpA could therefore influence the competition between principal and alternative sigma factors and hence the transcription profile of the cell.
DOI Link: 10.1074/jbc.M113.459883
ISSN: 0021-9258
eISSN: 1083-351X
Links: http://www.jbc.org/content/288/20/14438
http://hdl.handle.net/2381/28103
Version: Post-print
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2013, American Society for Biochemistry and Molecular Biology. Deposited with reference to the publisher’s archiving policy available on the SHERPA/RoMEO website.
Description: This research was originally published in The Journal of Biological Chemistry. Bortoluzzi, A., Muskett, F.W. et al., Mycobacterium tuberculosis RNA Polymerase-binding Protein A (RbpA) and Its Interactions with Sigma Factors, The Journal of Biological Chemistry, 2013, 288 (20), pp. 14438-14450, © the American Society for Biochemistry and Molecular Biology.
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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