Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/28498
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dc.contributor.authorMoiseeva, Elena P.-
dc.contributor.authorLeyland, Mark L.-
dc.contributor.authorBradding, Peter-
dc.date.accessioned2013-12-09T15:04:13Z-
dc.date.available2013-12-09T15:04:13Z-
dc.date.issued2012-10-11-
dc.identifier.citationMolecular Immunology, 2013, 53 (4), pp. 345-354en
dc.identifier.issn0161-5890-
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0161589012003975#en
dc.identifier.urihttp://hdl.handle.net/2381/28498-
dc.description.abstractCell adhesion molecule 1 (CADM1) is implicated in the pathogenesis of several diseases and is responsible for adhesion and survival of mast cells (MCs). Differential expression of CADM1 isoforms was found in different species. We previously cloned SP4, SP1, SP6 and a dysfunctional isoform from human lung MCs (HLMCs) and the MC line HMC-1. The aim of this study was to identify all isoforms expressed in human MCs. The functional isoforms SP4, SP1, SP6 and SP3, with alternative splicing between exons 7/11, were detected in human MCs by RT-PCR. Two dysfunctional isoforms with alternative splicing of cryptic exons A and B between exons 1/2, leading to premature termination of translation, were found in ∼40% of MC specimens. Sequencing of genomic DNA showed that splicing of cryptic exon B did not result from specific SNPs within this exon or its putative splice branch point. Highly glycosylated CADM1 (∼105 kDa) was detected by western blotting, but an extracellular domain (∼95 kDa) was found only in the culture medium from HLMCs, but not HMC-1 cells, indicating differential protein expression. Transfection of SP1 and SP6, but not SP4, reduced adhesion of HMC-1 cells to human lung fibroblasts but not airway smooth muscle cells. Hence, dysfunctional and functional CADM1 isoforms are found in human MCs. The longer SP1 and SP6 were most evident in differentiated HLMCs and displayed differential adhesion compared to SP4. These multiple isoforms are likely to contribute to MC function in both health and disease.en
dc.description.sponsorshipThis work is supported by Project Grant no. 87834 from the Medical Research Council, UK (P.B. and M. L. L.) and was conducted in laboratories part-funded by European Research Development Fund #05567.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/23063768-
dc.rightsCopyright © the authors, 2013. This is an open-access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/3.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.-
dc.subjectAlternative Splicingen
dc.subjectBase Sequenceen
dc.subjectCell Adhesionen
dc.subjectCell Adhesion Moleculesen
dc.subjectCell Differentiationen
dc.subjectCells, Cultureden
dc.subjectExonsen
dc.subjectFibroblastsen
dc.subjectGene Expression Regulationen
dc.subjectGlycosylationen
dc.subjectHumansen
dc.subjectImmunoglobulinsen
dc.subjectLungen
dc.subjectMast Cellsen
dc.subjectMolecular Sequence Dataen
dc.subjectPeptide Chain Termination, Translationalen
dc.subjectProtein Isoformsen
dc.subjectSpecies Specificityen
dc.subjectTransfectionen
dc.titleCADM1 is expressed as multiple alternatively spliced functional and dysfunctional isoforms in human mast cellsen
dc.typeJournal Articleen
dc.identifier.doi10.1016/j.molimm.2012.08.024-
dc.identifier.eissn1872-9142-
dc.identifier.piiS0161-5890(12)00397-5-
dc.description.statusPeer-reviewed-
dc.description.versionPublisher Version-
pubs.organisational-group/Organisationen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGYen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Biological Sciencesen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Biological Sciences/Department of Biochemistryen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicineen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Infection, Immunity and Inflammationen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themesen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themes/Molecular & Cellular Bioscienceen
pubs.organisational-group/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/Themes/Respiratory Scienceen
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

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