Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/28640
Title: Paroxysmal extreme pain disorder M1627K mutation in human Na[subscript v]1.7 renders DRG neurons hyperexcitable
Authors: Dib-Hajj, Sulayman D.
Estacion, Mark
Jarecki, Brian W.
Tyrrell, Lynda
Fischer, Tanya Z.
Lawden, Mark
Cummins, Theodore R.
Waxman, Stephen G.
First Published: 19-Sep-2008
Publisher: BioMed Central Ltd
Citation: Molecular Pain, 2008, 4 : 37
Abstract: Background: Paroxysmal extreme pain disorder (PEPD) is an autosomal dominant painful neuropathy with many, but not all, cases linked to gain-of-function mutations in SCN9A which encodes voltage-gated sodium channel Na[subscript v]1.7. Severe pain episodes and skin flushing start in infancy and are induced by perianal probing or bowl movement, and pain progresses to ocular and mandibular areas with age. Carbamazepine has been effective in relieving symptoms, while other drugs including other anti-epileptics are less effective. Results: Sequencing of SCN9A coding exons from an English patient, diagnosed with PEPD, has identified a methionine 1627 to lysine (M1627K) substitution in the linker joining segments S4 and S5 in domain IV. We confirm that M1627K depolarizes the voltage-dependence of fast-inactivation without substantially altering activation or slow-inactivation, and inactivates from the open state with slower kinetics. We show here that M1627K does not alter development of closed-state inactivation, and that M1627K channels recover from fast-inactivation faster than wild type channels, and produce larger currents in response to a slow ramp stimulus. Using current-clamp recordings, we also show that the M1627K mutant channel reduces the threshold for single action potentials in DRG neurons and increases the number of action potentials in response to graded stimuli. Conclusion: M1627K mutation was previously identified in a sporadic case of PEPD from France, and we now report it in an English family. We confirm the initial characterization of mutant M1627K effect on fast-inactivation of Na[subscript v]1.7 and extend the analysis to other gating properties of the channel. We also show that M1627K mutant channels render DRG neurons hyperexcitable. Our new data provide a link between altered channel biophysics and pain in PEPD patients.
DOI Link: 10.1186/1744-8069-4-37
ISSN: 1744-8069
eISSN: 1744-8069
Links: http://www.molecularpain.com/content/4/1/37
http://hdl.handle.net/2381/28640
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2008 Dib-Hajj et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Appears in Collections:Published Articles, College of Medicine, Biological Sciences and Psychology

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