Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/28701
Title: A simple method for the determination of reduction potentials in heme proteins
Authors: Efimov, Igor
Parkin, Gary
Millett, Elizabeth S.
Glenday, Jennifer
Chan, Cheuk K.
Weedon, Holly
Randhawa, Harpreet
Basran, Jaswir
Raven, Emma L.
First Published: 3-Mar-2014
Publisher: Elsevier on behalf of Federation of European Biochemical Societies
Citation: FEBS Letters, 2014, 588 (5), pp. 701-704
Abstract: We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (E[subscript m] = ˗115 mV ± 2 mV, pH 7.0) and human CLOCK (E[subscript m] = ˗111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.
DOI Link: 10.1016/j.febslet.2013.12.030
ISSN: 0014-5793
eISSN: 1873-3468
Links: http://www.sciencedirect.com/science/article/pii/S0014579314000283
http://hdl.handle.net/2381/28701
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2014 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Appears in Collections:Published Articles, Dept. of Chemistry

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