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Title: A simple method for the determination of reduction potentials in heme proteins
Authors: Efimov, Igor
Parkin, Gary
Millett, Elizabeth S.
Glenday, Jennifer
Chan, Cheuk K.
Weedon, Holly
Randhawa, Harpreet
Basran, Jaswir
Raven, Emma L.
First Published: 3-Mar-2014
Publisher: Elsevier on behalf of Federation of European Biochemical Societies
Citation: FEBS Letters, 2014, 588 (5), pp. 701-704
Abstract: We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (E[subscript m] = ˗115 mV ± 2 mV, pH 7.0) and human CLOCK (E[subscript m] = ˗111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.
DOI Link: 10.1016/j.febslet.2013.12.030
ISSN: 0014-5793
eISSN: 1873-3468
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: Copyright © 2014 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. This is an open-access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Appears in Collections:Published Articles, Dept. of Chemistry

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