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|Title:||Electrode calibration with a microfluidic flow cell for fast-scan cyclic voltammetry|
McCutcheon, James E.
Schuck, Matthew J.
Roitman, Mitchell F.
Eddington, David T.
|Publisher:||The Royal Society of Chemistry|
|Citation:||Lab on a Chip, 2012, 12 (13), pp. 2403-2403|
|Abstract:||Fast-scan cyclic voltammetry (FSCV) is a common analytical electrochemistry tool used to measure chemical species. It has recently been adapted for measurement of neurotransmitters such as dopamine in awake and behaving animals ( in vivo ). Electrode calibration is an essential step in FSCV to relate observed current to concentration of a chemical species. However, existing methods require multiple components, which reduce the ease of calibrations. To this end, a microfluidic flow cell ( m FC) was developed as a simple device to switch between buffer and buffer with a known concentration of the analyte of interest – in this case dopamine – in a microfluidic Y-channel. The ability to quickly switch solutions yielded electrode calibrations with faster rise times and that were more stable at peak current values. The m FC reduced the number of external electrical components and produced linear calibrations over a range of concentrations. To demonstrate this, an electrode calibrated with the m FC was used in FSCV recordings from a rat during the delivery of food reward – a stimulus that reliably evokes a brief increase in current due to the oxidation of dopamine. Using the linear calibration, dopamine concentrations were determined from the current responses evoked during the behavioral task. The m FC is able to easily and quickly calibrate FSCV electrode responses to chemical species for both in vitro and in vivo experiments.|
|Rights:||Copyright © 2012, Royal Society of Chemistry. Deposited with reference to the publisher’s open access archiving policy.|
|Appears in Collections:||Published Articles, School of Psychology|
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|Sinkala_LabChip_2012_official.pdf||Published (publisher PDF)||653.15 kB||Adobe PDF||View/Open|
|Sinkala_SuppInfo.pdf||Published (publisher PDF)||160.63 kB||Adobe PDF||View/Open|
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