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|Title:||IL-9 is a key component of memory TH cell peanut-specific responses from children with peanut allergy|
|Authors:||Brough, Helen A.|
Cousins, David J.
Wong, Yuen Fei
Stephens, Alick C.
|Publisher:||Elsevier for American Academy of Allergy, Asthma and Immunology|
|Citation:||Journal of Allergy and Clinical Immunology, 2014, DOI: 10.1016/j.jaci.2014.06.032|
|Abstract:||Background : Differentiation between patients with peanut allergy (PA) and those with peanut sensitization (PS) who tolerate peanut but have peanut-specific IgE, positive skin prick test responses, or both represents a significant diagnostic difficulty. Previously, gene expression microarrays were successfully used to identify biomarkers and explore immune responses during PA immunotherapy. Objective : We aimed to characterize peanut-specific responses from patients with PA, subjects with PS, and atopic children without peanut allergy (NA children). Methods : A preliminary exploratory microarray investigation of gene expression in peanut-activated memory TH subsets from 3 children with PA and 3 NA children identified potential PA diagnostic biomarkers. Microarray findings were confirmed by using real-time quantitative PCR in 30 subjects (12 children with PA, 12 children with PS, and 6 NA children). Flow cytometry was used to identify the TH subsets involved. Results : Among 12,257 differentially expressed genes, IL9 showed the greatest difference between children with PA and NA children (45.59-fold change, P < .001), followed by IL5 and then IL13. Notably, IL9 allowed the most accurate classification of children with PA and NA children by using a machine-learning approach with recursive feature elimination and the random forest algorithm. Skin- and gut-homing TH cells from donors with PA expressed similar TH2- and TH9-associated genes. Real-time quantitative PCR confirmed that IL9 was the highest differentially expressed gene between children with PA and NA children (23.3-fold change, P < .01) and children with PS (18.5-fold change, P < .05). Intracellular cytokine staining showed that IL-9 and the TH2-specific cytokine IL-5 are produced by distinct TH populations. Conclusion : In this study IL9 best differentiated between children with PA and children with PS (and atopic NA children). Mutually exclusive production of IL-9 and the TH2-specific cytokine IL-5 suggests that the IL-9–producing cells belong to the recently described TH9 subset.|
|Rights:||Copyright © 2014, Elsevier. Deposited with reference to the publisher’s archiving policy available on the SHERPA/RoMEO website|
|Appears in Collections:||Published Articles, Dept. of Infection, Immunity and Inflammation|
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|Final submitted manuscript JACI postsubmission240514-1.pdf||Post-review (final submitted)||174.77 kB||Adobe PDF||View/Open|
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