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Title: CIM (R) monolithic anion-exchange chromatography as a useful alternative to CsCl gradient purification of bacteriophage particles
Authors: Adriaenssens, E. M.
Lehman, S. M.
Vandersteegen, K.
Vandenheuvel, D.
Philippe, Didier L.
Cornelissen, A.
Clokie, Martha R.J
Garcia, A. J.
De Proft, M.
Maes, M.
Lavigne, R.
First Published: 20-Dec-2012
Publisher: Academic Press from Elsevier Science
Citation: Virology, 2012, 434 (2), pp. 265-270 (6)
Abstract: The use of anion-exchange chromatography was investigated as an alternative method to concentrate and purify bacterial viruses, and parameters for different bacteriophages were compared. Chromatography was performed with Convective Interactive Media® monoliths, with three different volumes and two matrix chemistries. Eleven morphologically distinct phages were tested, infecting five different bacterial species. For each of the phages tested, a protocol was optimized, including the choice of column chemistry, loading, buffer and elution conditions. The capacity and recovery of the phages on the columns varied considerably between phages. We conclude that anion-exchange chromatography with monoliths is a valid alternative to the more traditional CsCl purification, has upscaling advantages, but it requires more extensive optimization.
DOI Link: 10.1016/j.virol.2012.09.018
ISSN: 0042-6822
Version: Post-print
Status: Peer-reviewed
Type: Journal Article
Rights: This is the author’s version of a work that was accepted for publication in Virology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Virology [Volume 434, Issue 2, 20 December 2012] DOI:10.1016/j.virol.2012.09.018
Appears in Collections:Published Articles, Dept. of Infection, Immunity and Inflammation

Files in This Item:
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paper CIM_Virology.pdfPost-review (final submitted)245.95 kBAdobe PDFView/Open
Table 1_JVM.pdfPost-review (final submitted)240.19 kBAdobe PDFView/Open
Table CsCl.pdfPost-review (final submitted)97.5 kBAdobe PDFView/Open
Figure 1.tifPost-review (final submitted)377.81 kBTIFFView/Open
Figure 2 color.tifPost-review (final submitted)239.27 kBTIFFView/Open
Figure 2 grayscale.tifPost-review (final submitted)175.04 kBTIFFView/Open

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