Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/31473
Title: Evidence of convergent evolution in humans and macaques supports an adaptive role for copy number variation of the β-defensin-2 gene.
Authors: Ottolini, Barbara
Hornsby, M. J.
Abujaber, Razan
MacArthur, J. A.
Badge, Richard M.
Schwarzacher, T.
Albertson, D. G.
Bevins, C. L.
Solnick, J. V.
Hollox, Edward J.
First Published: 27-Oct-2014
Publisher: Oxford University Press (OUP) For: Society for Molecular Biology and Evolution
Citation: Genome Biol Evol, 2014, 6 (11), pp. 3025-3038
Abstract: β-defensins are a family of important peptides of innate immunity, involved in host defense, immunomodulation, reproduction, and pigmentation. Genes encoding β-defensins show evidence of birth-and-death evolution, adaptation by amino acid sequence changes, and extensive copy number variation (CNV) within humans and other species. The role of CNV in the adaptation of β-defensins to new functions remains unclear, as does the adaptive role of CNV in general. Here, we fine-map CNV of a cluster of β-defensins in humans and rhesus macaques. Remarkably, we found that the structure of the CNV is different between primates, with distinct mutational origins and CNV boundaries defined by retroviral long terminal repeat elements. Although the human β-defensin CNV region is 322 kb and encompasses several genes, including β-defensins, a long noncoding RNA gene, and testes-specific zinc-finger transcription factors, the orthologous region in the rhesus macaque shows CNV of a 20-kb region, containing only a single gene, the ortholog of the human β-defensin-2 gene. Despite its independent origins, the range of gene copy numbers in the rhesus macaque is similar to humans. In addition, the rhesus macaque gene has been subject to divergent positive selection at the amino acid level following its initial duplication event between 3 and 9.5 Ma, suggesting adaptation of this gene as the macaque successfully colonized novel environments outside Africa. Therefore, the molecular phenotype of β-defensin-2 CNV has undergone convergent evolution, and this gene shows evidence of adaptation at the amino acid level in rhesus macaques.
DOI Link: 10.1093/gbe/evu236
ISSN: 1759-6653
eISSN: 1759-6653
Links: http://gbe.oxfordjournals.org/content/6/11/3025
http://hdl.handle.net/2381/31473
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: © The Author(s) 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Description: PMCID: PMC4255768
Appears in Collections:Published Articles, Dept. of Genetics

Files in This Item:
File Description SizeFormat 
Genome Biol Evol-2014-Ottolini-3025-38.pdfPublished (publisher PDF)1.28 MBAdobe PDFView/Open


Items in LRA are protected by copyright, with all rights reserved, unless otherwise indicated.