Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/31611
Title: Evaluating the translational potential of progesterone treatment following transient cerebral ischaemia in male mice.
Authors: Wong, R.
Gibson, Claire L.
Kendall, D. A.
Bath, P. M.
First Published: 29-Nov-2014
Publisher: BioMed Central, United Kingdom
Citation: Wong et al.: Evaluating the translational potential of progesterone treatment following transient cerebral ischaemia in male mice. BMC Neuroscience 2014 15:131.
Abstract: Background: Progesterone is neuroprotective in numerous preclinical CNS injury models including cerebral ischaemia. The aim of this study was two-fold; firstly, we aimed to determine whether progesterone delivery via osmotic mini-pump would confer neuroprotective effects and whether such neuroprotection could be produced in co-morbid animals. Results: Animals underwent transient middle cerebral artery occlusion. At the onset of reperfusion, mice were injected intraperitoneally with progesterone (8 mg/kg in dimethylsulfoxide). Adult and aged C57 Bl/6 mice were dosed additionally with subcutaneous infusion (1.0 μl/h of a 50 mg/ml progesterone solution) via implanted osmotic minipumps. Mice were allowed to survive for up to 7 days post-ischaemia and assessed for general well-being (mass loss and survival), neurological score, foot fault and t-maze performance. Progesterone reduced neurological deficit [F(1,2) = 5.38, P = 0.027] and number of contralateral foot-faults [F(1,2) = 7.36, P = 0.0108] in adult, but not aged animals, following ischaemia. In hypertensive animals, progesterone treatment lowered neurological deficit [F(1,6) = 18.31, P = 0.0001], reduced contralateral/ipsilateral alternation ratio % [F(1,2) = 17.05, P = 0.0006] and time taken to complete trials [F(1,2) = 15.92, P = 0.0009] for t-maze. Conclusion: Post-ischemic progesterone administration via mini-pump delivery is effective in conferring functional improvement in a transient MCAO model in adult mice. Preliminary data suggests such a treatment regimen was not effective in producing a protective effect in aged mice. However, in hypertensive mice, who received post-ischemic progesterone intraperitoneally at the onset of reperfusion had better functional outcomes than control hypertensive mice.
DOI Link: 10.1186/s12868-014-0131-5
ISSN: 1471-2202
eISSN: 1471-2202
Links: http://www.biomedcentral.com/1471-2202/15/131
http://hdl.handle.net/2381/31611
Version: Publisher Version
Status: Peer-reviewed
Type: Journal Article
Rights: © 2014 Wong et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Description: PMCID: PMC4255926
Appears in Collections:Published Articles, School of Psychology

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