Please use this identifier to cite or link to this item: http://hdl.handle.net/2381/31710
Title: BURSTING POLLEN is required to organize the pollen germination plaque and pollen tube tip in Arabidopsis thaliana.
Authors: Hoedemaekers, Karin
Derksen, Jan
Hoogstrate, Suzanne W.
Wolters-Arts, Mieke
Oh, Sung-Aeong
Twell, David
Mariani, Celestina
Rieu, Ivo
First Published: 1-Dec-2014
Publisher: Wiley for New Phytologist Trust
Citation: Hoedemaekers, K., Derksen, J., Hoogstrate, S. W., Wolters-Arts, M., Oh, S.-A., Twell, D., Mariani, C. and Rieu, I. (2014), BURSTING POLLEN is required to organize the pollen germination plaque and pollen tube tip in Arabidopsis thaliana. New Phytologist.
Abstract: Pollen germination may occur via the so-called germination pores or directly through the pollen wall at the site of contact with the stigma. In this study, we addressed what processes take place during pollen hydration (i.e. before tube emergence), in a species with extra-poral pollen germination, Arabidopsis thaliana. A T-DNA mutant population was screened by segregation distortion analysis. Histological and electron microscopy techniques were applied to examine the wild-type and mutant phenotypes. Within 1 h of the start of pollen hydration, an intine-like structure consisting of cellulose, callose and at least partly de-esterified pectin was formed at the pollen wall. Subsequently, this 'germination plaque' gradually extended and opened up to provide passage for the cytoplasm into the emerging pollen tube. BURSTING POLLEN (BUP) was identified as a gene essential for the correct organization of this plaque and the tip of the pollen tube. BUP encodes a novel Golgi-located glycosyltransferase related to the glycosyltransferase 4 (GT4) subfamily which is conserved throughout the plant kingdom. Extra-poral pollen germination involves the development of a germination plaque and BUP defines the correct plastic-elastic properties of this plaque and the pollen tube tip by affecting pectin synthesis or delivery.
DOI Link: 10.1111/nph.13200
ISSN: 0028-646X
eISSN: 1469-8137
Links: http://onlinelibrary.wiley.com/doi/10.1111/nph.13200/abstract
http://hdl.handle.net/2381/31710
Version: Post-print
Status: Peer-reviewed
Type: Journal Article
Rights: Archived with reference to SHERPA/RoMEO and publisher website. This is the accepted version of the following article: Hoedemaekers, K., Derksen, J., Hoogstrate, S. W., Wolters-Arts, M., Oh, S.-A., Twell, D., Mariani, C. and Rieu, I. (2014), BURSTING POLLEN is required to organize the pollen germination plaque and pollen tube tip in Arabidopsis thaliana. New Phytologist., which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/nph.13200/abstract
Appears in Collections:Published Articles, Dept. of Biology

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